| 质粒名称: | pVITRO-HPV11 L1L2 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 52590 |
| 存储实验室: | Richard Roden |
|
载体骨架基本信息 |
|
| 载体名称: | pVITRO1-neo-mcs |
| 载体出品公司: | InvivoGen |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 6295 bp |
| 完整质粒大小: | 9175 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | HPV11 L1 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 1509 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BglII（在克隆过程中被销毁） |
| 3' 克隆位点: | NheI（在克隆过程中被销毁） |
| 5' 测序引物 | AAACCACCGCTAATTCAAAGC |
| 3' 测序引物 | GTGGTTTGTCCAAACTCATCAA |
|
文献引用方法 |
|
| 材料和方法部分: | pVITRO-HPV11 L1L2 was a gift from Richard Roden (Addgene plasmid # 52590 ; http://n2t.net/addgene:52590 ; RRID:Addgene_52590) |
| 参考文献部分: | Impact of inhibitors and L2 antibodies upon the infectivity of diverse alpha and beta human papillomavirus types. Kwak K, Jiang R, Wang JW, Jagu S, Kirnbauer R, Roden RB. PLoS One. 2014 May 9;9(5):e97232. doi: 10.1371/journal.pone.0097232. eCollection 2014. 10.1371/journal.pone.0097232 PubMed 24816794 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pVITRO-HPV11 L1L2 9175 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expresses HPV11 L1 and L2 in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pVITRO-HPV11 L1L2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9175)
AUTHORS Kwak K, Jiang R, Wang JW, Jagu S, Kirnbauer R, Roden RB
TITLE Impact of inhibitors and L2 antibodies upon the infectivity of
diverse alpha and beta human papillomavirus types.
JOURNAL PLoS One. 2014 May 9;9(5):e97232. doi: 10.1371/journal.pone.0097232.
eCollection 2014.
PUBMED 24816794
REFERENCE 2 (bases 1 to 9175)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9175
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(74..94)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
intron 583..1530
/label=mEF-1-alpha intron
/note="intron upstream of the start codon of mouse
EF-1-alpha"
polyA_signal 3116..3237
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3127..3146)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 3181..3200
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
rep_origin 3425..4013
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 3914..3933
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
enhancer 4093..4396
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
misc_feature 7218..7662
/label=FMDV IRES
/note="internal ribosome entry site (IRES) of the
foot-and-mouth disease virus"
promoter 7695..7741
/label=EM7 promoter
/note="synthetic bacterial promoter "
promoter 7733..7751
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 7761..8555
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(7815..7834)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 8425..8444
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 8602..9174
/label=EF-1-alpha poly(A) signal
/note="human EF-1-alpha polyadenylation signal"
ORIGIN
1 cctgcagggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga
61 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
121 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
181 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
241 ccactagtgg agccgagagt aattcataca aaaggaggga tcgccttcgc aaggggagag
301 cccagggacc gtccctaaat tctcacagac ccaaatccct gtagccgccc cacgacagcg
361 cgaggagcat gcgctcaggg ctgagcgcgg ggagagcaga gcacacaagc tcatagaccc
421 tggtcgtggg ggggaggacc ggggagctgg cgcggggcaa actgggaaag cggtgtcgtg
481 tgctggctcc gccctcttcc cgagggtggg ggagaacggt atataagtgc ggcagtcgcc
541 ttggacgttc tttttcgcaa cgggtttgcc gtcagaacgc aggtgagggg cgggtgtggc
601 ttccgcgggc cgccgagctg gaggtcctgc tccgagcggg ccgggccccg ctgtcgtcgg
661 cggggattag ctgcgagcat tcccgcttcg agttgcgggc ggcgcgggag gcagagtgcg
721 aggcctagcg gcaaccccgt agcctcgcct cgtgtccggc ttgaggccta gcgtggtgtc
781 cgcgccgccg ccgcgtgcta ctccggccgc actctggtct tttttttttt tgttgttgtt
841 gccctgctgc cttcgattgc cgttcagcaa taggggctaa caaagggagg gtgcggggct
901 tgctcgcccg gagcccggag aggtcatggt tggggaggaa tggagggaca ggagtggcgg
961 ctggggcccg cccgccttcg gagcacatgt ccgacgccac ctggatgggg cgaggcctgg
1021 ggtttttccc gaagcaacca ggctggggtt agcgtgccga ggccatgtgg ccccagcacc
1081 cggcacgatc tggcttggcg gcgccgcgtt gccctgcctc cctaactagg gtgaggccat
1141 cccgtccggc accagttgcg tgcgtggaaa gatggccgct cccgggccct gttgcaagga
1201 gctcaaaatg gaggacgcgg cagcccggtg gagcgggcgg gtgagtcacc cacacaaagg
1261 aagagggcct ggtccctcac cggctgctgc ttcctgtgac cccgtggtcc tatcggccgc
1321 aatagtcacc tcgggctttt gagcacggct agtcgcggcg gggggagggg atgtaatggc
1381 gttggagttt gttcacattt ggtgggtgga gactagtcag gccagcctgg cgctggaagt
1441 catttttgga atttgtcccc ttgagttttg agcggagcta attctcgggc ttcttagcgg
1501 ttcaaaggta tcttttaaac ccttttttag gtgttgtgaa aaccaccgct aattcaaagc
1561 aaccggtgat atcaaagatc cgccaccatg tggcgaccct cagatagcac tgtatacgtc
1621 cccccgccaa accccgtcag caaggttgta gcgaccgacg cctacgtcaa gaggaccaat
1681 attttctatc acgccagctc aagtaggttg ctggcagtcg gccatccata ctattcaatt
1741 aagaaagtta acaagactgt ggtgccgaaa gtgagcggct accagtacag agtatttaaa
1801 gtcgtattgc cggaccccaa caagttcgcc ctccctgatt cttctctttt tgatcccact
1861 acgcagcggt tggtatgggc ttgcaccggg ctcgaagtgg gacgggggca gcctctcggc
1921 gtgggcgtga gcgggcatcc cctgctgaac aagtacgatg acgtggagaa ttctggtggg
1981 tatggcggta acccaggcca ggataatcgc gtgaatgtgg gcatggacta caagcagact
2041 cagctgtgta tggtgggctg cgctccacct ctgggggaac actggggcaa gggaacccag
2101 tgcagcaata catccgtgca gaacggcgac tgtcctccac ttgaactcat cacgagcgtc
2161 atccaggacg gagatatggt ggatacaggc tttggtgcaa tgaatttcgc cgacctccag
2221 acaaacaaga gtgacgtgcc tcttgacatt tgtggaactg tttgcaaata ccctgactat
2281 cttcagatgg ctgctgaccc ctatggagat aggcttttct tttatctgcg gaaagaacag
2341 atgttcgcca gacatttctt taaccgggca gggaccgttg gggagcccgt tcccgacgat
2401 ctcttggtga aagggggaaa caaccggagt tccgtcgcct ccagtatcta tgtgcataca
2461 ccttccggta gcctggtttc ctccgaggcc cagctgttca acaaaccata ttggctccag
2521 aaagcgcaag gtcacaataa cgggatatgt tggggaaatc acttgtttgt gaccgttgtg
2581 gatactaccc gctctaccaa tatgaccctg tgcgcttctg tcagcaagtc cgcaacatat
2641 acaaattccg actacaaaga gtatatgaga cacgtggagg agtttgactt gcaattcatt
2701 ttccaactgt gctccatcac tctgagtgct gaggtcatgg cctacataca caccatgaac
2761 ccttctgtgc tggaagactg gaatttcggg ctcagtccac ctccaaacgg cacactcgag
2821 gatacgtata gatacgttca aagccaggcc atcacatgtc agaaacctac ccccgaaaag
2881 gagaagcagg acccatacaa ggatatgtca ttttgggaag tcaatctgaa ggaaaagttc
2941 agctctgagc tggatcaatt cccactgggc cgcaagtttc ttctgcaaag tggatacagg
3001 ggaagaactt cagcacgcac cggaatcaaa cgacccgccg tgtcaaagcc gtcaacagca
3061 cccaaaagga agcggacgaa aacaaagaaa taatagtcta gctggccaga catgataaga
3121 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
3181 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
3241 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
3301 agcaagtaaa acctctacaa atgtggtatg gaaatgttaa ttaactagcc atgaccaaaa
3361 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
3421 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
3481 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
3541 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
3601 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
3661 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
3721 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
3781 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
3841 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
3901 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
3961 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
4021 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttcttaa
4081 ttaacctgca ggcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
4141 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
4201 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
4261 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
4321 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
4381 catcgctatt accatgatga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
4441 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttgact
4501 agtggagccg agagtaattc atacaaaagg agggatcgcc ttcgcaaggg gagagcccag
4561 ggaccgtccc taaattctca cagacccaaa tccctgtagc cgccccacga cagcgcgagg
4621 agcatgcgcc cagggctgag cgcgggtaga tcagagcaca caagctcaca gtccccggcg
4681 gtggggggag gggcgcgctg agcgggggcc agggagctgg cgcggggcaa actgggaaag
4741 tggtgtcgtg tgctggctcc gccctcttcc cgagggtggg ggagaacggt atataagtgc
4801 ggtagtcgcc ttggacgttc tttttcgcaa cgggtttgcc gtcagaacgc aggtgagtgg
4861 cgggtgtggc ttccgcgggc cccggagctg gagccctgct ctgagcgggc cgggctgata
4921 tgcgagtgtc gtccgcaggg tttagctgtg agcattccca cttcgagtgg cgggcggtgc
4981 gggggtgaga gtgcgaggcc tagcggcaac cccgtagcct cgcctcgtgt ccggcttgag
5041 gcctagcgtg gtgtccgccg ccgcgtgcca ctccggccgc actatgcgtt ttttgtcctt
5101 gctgccctcg attgccttcc agcagcatgg gctaacaaag ggagggtgtg gggctcactc
5161 ttaaggagcc catgaagctt acgttggata ggaatggaag ggcaggaggg gcgactgggg
5221 cccgcccgcc ttcggagcac atgtccgacg ccacctggat ggggcgaggc ctgtggcttt
5281 ccgaagcaat cgggcgtgag tttagcctac ctgggccatg tggccctagc actgggcacg
5341 gtctggcctg gcggtgccgc gttcccttgc ctcccaacaa gggtgaggcc gtcccgcccg
5401 gcaccagttg cttgcgcgga aagatggccg ctcccggggc cctgttgcaa ggagctcaaa
5461 atggaggacg cggcagcccg gtggagcggg cgggtgagtc acccacacaa aggaagaggg
5521 ccttgcccct cgccggccgc tgcttcctgt gaccccgtgg tctatcggcc gcatagtcac
5581 ctcgggcttc tcttgagcac cgctcgtcgc ggcgggggga ggggatctaa tggcgttgga
5641 gtttgttcac atttggtggg tggagactag tcaggccagc ctggcgctgg aagtcattct
5701 tggaatttgc ccctttgagt ttggagcgag gctaattctc aagcctctta gcggttcaaa
5761 ggtattttct aaacccgttt ccaggtgttg tgaaagccac cgctaattca aagcaatccg
5821 gagtatacgg atccgccacc atgaaaccca gagccagaag aagaaagagg gcatcagcaa
5881 cacagctgta ccagacctgt aaggcgacag gcacctgccc gccagacgtg attcctaaag
5941 tagagcacac cactatagcc gaccagattt tgaagtgggg gtccctcggc gtgtttttcg
6001 gcggtctcgg gatcggaacc ggagctgggt ctggtggcag agcaggatac attcctctgg
6061 gctcttcccc caagcccgca attacagggg gtccagccgc taggcctccc gtcctggtgg
6121 agcctgtggc cccgtccgac cccagtacag tgagtttgat agaagagtcc gcaatcatca
6181 acgccggcgc ccctgaagtt gtcccaccca cacagggagg cttcaccatc acttcctcag
6241 aaagtactac tccggctatt ctggacgtgt ctgtgaccaa ccatacaacc acctctgtct
6301 tccagaatcc cctctttact gagccgagtg ttattcagcc ccagccccca gtcgaagcct
6361 caggacacat cttgatctca gctccaacga tcaccagcca gcatgtcgag gacatccccc
6421 ttgacacatt tgtcgtgtca agcagtgata gtggcccgac ttcctccact cctctgcccc
6481 gcgccttccc tcgcccccgg gtgggtcttt attctcgggc ccttcaacaa gtacaggtta
6541 ctgatccagc gttcctgagc acaccacagc gcctcgtgac gtacgataat cctgtgtacg
6601 agggggagga tgtttccctg caatttaccc acgagagcat ccataatgct cctgatgagg
6661 ctttcatgga tattatacgc ttgcaccggc cagccatcac atcaaggaga ggtttggtgc
6721 gattcagccg aatcggccag aggggcagca tgtacacccg gtctgggcag cacatcgggg
6781 ctcggattca ctattttcaa gacatctctc ctgtcactca agcggctgaa gaaatcgaac
6841 tgcatccact ggtcgctgca gagaacgata cctttgatat ttacgccgaa ccttttgatc
6901 caataccgga cccagtgcag catagcgtga cgcagtctta tctcacgagt acacctaaca
6961 cactttctca gtcatggggg aataccacag ttcctctctc catacccagc gactggtttg
7021 tacagagcgg accagacatt actttcccca ccgccagcat gggcacccct ttctcccccg
7081 taacccctgc actgccaact ggaccagttt tcatcacagg aagcgatttt tatcttcacc
7141 ccacgtggta tttcgccagg cgaaggcgca aacggattcc cctgtttttc accgacgtgg
7201 cagcctaata gtctaggagc aggtttcccc aatgacacaa aacgtgcaac ttgaaactcc
7261 gcctggtctt tccaggtcta gaggggtaac actttgtact gcgtttggct ccacgctcga
7321 tccactggcg agtgttagta acagcactgt tgcttcgtag cggagcatga cggccgtggg
7381 aactcctcct tggtaacaag gacccacggg gccaaaagcc acgcccacac gggcccgtca
7441 tgtgtgcaac cccagcacgg cgactttact gcgaaaccca ctttaaagtg acattgaaac
7501 tggtacccac acactggtga caggctaagg atgcccttca ggtaccccga ggtaacacgc
7561 gacactcggg atctgagaag gggactgggg cttctataaa agcgctcggt ttaaaaagct
7621 tctatgcctg aataggtgac cggaggtcgg cacctttcct ttgcaattac tgaccctatg
7681 aatacactga ctgtttgaca attaatcatc ggcatagtat atcggcatag tataatacga
7741 ctcactatag gagggccacc atgattgaac aagatggatt gcacgcaggt tctccggccg
7801 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
7861 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
7921 ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg
7981 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
8041 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
8101 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
8161 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
8221 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
8281 tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac acatggcgat gcctgcttgc
8341 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
8401 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
8461 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
8521 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
8581 cgaccaagcg aattcgctag gattatccct aatacctgcc accccactct taatcagtgg
8641 tggaagaacg gtctcagaac tgtttgtttc aattggccat ttaagtttag tagtaaaaga
8701 ctggttaatg ataacaatgc atcgtaaaac cttcagaagg aaaggagaat gttttgtgga
8761 ccactttggt tttctttttt gcgtgtggca gttttaagtt attagttttt aaaatcagta
8821 ctttttaatg gaaacaactt gaccaaaaat ttgtcacaga attttgagac ccattaaaaa
8881 agttaaatga gaaacctgtg tgttcctttg gtcaacaccg agacatttag gtgaaagaca
8941 tctaattctg gttttacgaa tctggaaact tcttgaaaat gtaattcttg agttaacact
9001 tctgggtgga gaatagggtt gttttccccc cacataattg gaaggggaag gaatatcatt
9061 taaagctatg ggagggttgc tttgattaca acactggaga gaaatgcagc atgttgctga
9121 ttgcctgtca ctaaaacagg ccaaaaactg agtccttggg ttgcatagaa agctg
//
